人工脂質膜重組電生理系統

• 詳細介紹
• 詳細參數

• 詳細介紹

  德國Ionovation公司生產的Ionovation scout人工脂質膜重組電生理分析系統，是該領域最新的產品之一，該產品克服了傳統膜片鉗的一系列缺點，無論從產品的技術含量還是從產品的應用領域上來看，在電生理分析技術中始終處于領先的地位，代表著電生理分析技術發展的方向，是國內外細胞電生理分析實驗室首選實驗儀器。這種高度靈活的桌面檢測系統可適應多種實驗條件，目前已經被用于對細胞膜離子通道、多種動物和植物轉運蛋白和它們的細胞器進行研究。

  Ionovation scout 技術特點：

  √ 不需要建立千兆歐姆的封阻

  √ 是唯一一種采用雙層封阻的測量方式

  √ 可用于檢測各種細胞膜上的離子通道、囊泡、配體

  √容易實現對單個離子通道進行分析檢測

  √可以在膜的兩側改變條件，形成雙信道進行分析              

  
  

  
  

  
  

  
  

  技術原理：

  
  

  Ionovation Scout采用了人工生物脂質膜來構建離子通道的測量方法。該系統使用兩個獨立的電生理測量室進行測量。該雙層室由一個25μm厚、直徑120微米的聚四氟乙烯隔膜隔開（a）。兩個聚碳酸酯小室的體積分別為1.2 ml。聚碳酸酯小室頂部有孔，方便接入的Ag / AgCl電極和注入緩沖液（a）。當兩個腔室中填充有合適的緩沖液時，溶解在正癸烷的脂質被分散到直徑120微米的特氟隆隔膜的微孔（ b）中。緩沖液的液面經過連續地降低和升高過程，直到特氟隆隔膜上的過量脂質被除去，特氟隆隔膜上形成脂雙層自組織形成的人工生物脂質膜（C）。

  然后根據具體的目的蛋白或者細胞器等進行操作。比如類似于α-溶血素的毒素，可以直接加入聚碳酸酯小室中進行測試。而需要重組的離子通道則需要將其與脂肪酸結合為重組脂蛋白，然后將這些脂蛋白或者從生物膜上“剪下”囊泡與人工生物脂質膜通過離子梯度或融合肽方法進行融合。

  準備好樣品后，開始進行電生理學測試。通過施加不同的電壓，來監測細胞單離子通道的電流，其中在5-10 kHz帶寬的信號會存儲在系統的硬盤上，測試通常采用的模式是電壓鉗模式（voltage clamp mode），這種模式下應用不同的電壓來得到目的信號。

  
  

  應用領域：

  1.脂質觸發器

  2.細胞膜轉運處理

  3.膜活性劑比如突觸核蛋白

  4.藥物安全分析

  5.信號轉導模式分析

  
  

  
  

  
  

  應用案例

  Ionovation Scout測試系統可用于多種離子通道的分析:

  
  

  1. 測量對象：可實現對上萬種離子通道、轉運體和孔隙活動的精確測量；

  2. 軟件：電生理數據采集分析軟件Patchmaster，采用用戶界面友好的軟件實現測量全部控制；具備預定義協議式的用戶定義實驗工作流程；

  3. 自動操作：全自動設備操作（雙層生產和驗證、雙層完整性的電容控制、膜雙側灌注系統）；雙層分子可視化操作；

  4. 電流測量：穩定低噪聲Ag/AgCl電極，采用鹽橋記錄電流范圍從pA到幾個nA

  5. 耗材：一次性雙分子層室可簡單、快速置換；

  
  

• 詳細參數

  OVERVIEW:

   Ionovation company Ionovation scout restructuring of bilayer lipid membrane electrophysiological analysis system, is one of the latest products in this field, it overcomes the traditional patch clamp series of shortcomings, whether from the technical content of products or from the perspective of the application field of the product, in the electrophysiological analysis technology has always been in a leading position, the developing direction of the electrophysiological analysis technology, is the preferred cell electrophysiological analysis laboratory experimental apparatus at home and abroad. This highly flexible desktop detection system is adapted to a variety of experimental conditions and has been used to study cell membrane ion channels, various animal and plant transporters and their organelles.

  Ionovation scout technical characteristics：

  √ don't need to create a gigaom block

  √ the only way to measure the double seal

  √ can be used to detect ion channels, vesicles and ligands on various cell membranes

  √easy to carry out analysis and detection for single ion channel

  √The condition can be changed on both sides of the membrane to form a double channel for analysis              

  
  

  Technology principle:

  
  

        The Ionovation Scout USES artificial lipid membranes to construct ion channels. The system is measured using two separate electrophysiological measurement Chambers. The double chamber is separated (a) by a polytetrafluoroethylene septum (a) 25 mm thick and 120 micron in diameter. The volume of the two polycarbonate cells was 1.2ml. The polycarbonate chamber has holes at the top for easy access to Ag/AgCl electrodes and injection buffer (a). When the two Chambers are filled with the appropriate buffer, the lipids dissolved in the n-decane are dispersed into the pore (b) of the teflon diaphragm, which is 120 microns in diameter. The level of the buffer is continuously lowered and elevated until the excess lipids on the teflon diaphragm are removed, and an artificial biolipid membrane (C) is formed on the teflon diaphragm with a double layer of lipid self-tissue.  

        It is then manipulated according to specific target proteins or organelles. For example, a toxin similar to the hemolysin can be added directly to the polycarbonate chamber for testing. The recombinant ion channel needs to be combined with fatty acids to form recombinant lipoproteins, and these lipoproteins are then "cut" from the biofilm to fuse with the artificial lipid membrane by ion gradient or fusion peptide method.

  After the sample was prepared, the electrophysiology test was carried out. By applying different voltages to monitor the current in the cell's single ion channel, where the signal with a bandwidth of 5-10 kHz is stored on the system's hard drive, the test usually takes the form of voltage clamp mode, in which different voltages are applied to get the target signal.

  
  

  Application：

  1.Lipid triggers

  2.Cell membrane transport

  3.Membrane active agents such as synaptic nucleoprotein

  4.Drug safety analysis

  5.Signal transduction mode analysis

  
  

  Application case

  Ionovation Scout test system can be used for analysis of multiple ion channels:

  1. Measuring objects: accurate measurement of thousands of ion channels, transporters and pore activities can be achieved.

  2. Software: electrophysiological data collection and analysis software Patchmaster, which USES user-friendly software to realize all measurement control; User defined experimental workflow with pre-defined protocols;

  3. Automatic operation: full-automatic equipment operation (double-layer production and verification, double-layer integrity of capacitance control, film two-side perfusion system); Two layer visualization operation;

  4. Current measurement: stable low noise Ag/AgCl electrode, using salt bridge to record the current range from pA to several nA

  5. Consumables: one-time bimolecular chamber can be simply and quickly replaced;

  
  

• 產品特色

  因用于機器人各方面應用且與大多數機器人類型兼容，AutoCal系統可以檢測出機器人自身構造和工具中心點（TCP）的 突然改變或偏離，并且該系統無需人為干涉就自動地更正這些誤差。

  AutoCal系統-Dynalog的先進水平校準技術，Dynalog是機器人單元標定技術的世界領導者。它的主流產品DynaCal 系統，被應用于離線的機器人單元校準，并作為最精確的和技術先進的機器人校準程序為許多機器人制造商和終端使用者所接受。AutoCal 系統將已證實的DynaCal校準技術結合到一個在線的全自動系統中，該系統專為程序控制和復原而設計的，價格低廉。

• 詳細介紹

  AutoCal系統提供在線的機器人校準方案，旨在快速和自動地保證機械設備的工作性能。因用于機器人各方面應用且與大多數機器人類型兼容，AutoCal系統可以檢測出機器人自身構造和工具中心點（TCP）的 突然改變或偏離，并且該系統無需人為干涉就自動地更正這些誤差。這意味著不用猜測哪里會出錯，不用浪費寶貴時間在機器人程序重復校準上，產品品質無任何損失。

  AutoCal系統-Dynalog的先進水平校準技術，Dynalog是機器人單元標定技術的世界領導者。它的主流產品DynaCal 系統，被應用于離線的機器人單元校準，并作為最精確的和技術先進的機器人校準程序為許多機器人制造商和終端使用者所接受。AutoCal 系統將已證實的DynaCal校準技術結合到一個在線的全自動系統中，該系統專為程序控制和復原而設計的，價格低廉。